human renal epithelial cell line a498  (ATCC)

Journal: Cells

Article Title: Human Renal Fibroblasts, but Not Renal Epithelial Cells, Induce IL-1β Release during a Uropathogenic Escherichia coli Infection In Vitro

doi: 10.3390/cells10123522

Figure Lengend Snippet: IL-1β and LDH release during a UPEC infection. Renal fibroblasts (TK173) and renal epithelial cells (HK-2 and A498) were infected with UPEC strain CFT073 at MOI 1 or MOI 10 for 6 h followed by analysis of IL-1β ( A ) and LDH release ( B ). Data are presented as mean ± SEM ( n = 3 independent experiments). Asterisks over bars denote statistical significance compared to respective unstimulated control (*** p < 0.001).

Article Snippet: Human renal medullary fibroblast cell line TK173 [ ] (kind gift from Professor Anton Jan van Zonneveld, Leiden University, Leiden, Netherlands), human renal epithelial cell line A498 (HTB-44, American Type Culture Collection (ATCC), Manassas, VA, USA) and the human immortalized proximal tubule epithelial cell line HK-2 (ATCC) were used in this study.

Techniques: Infection, Control

Journal: Scientific reports

Article Title: Human renal fibroblasts are strong immunomobilizers during a urinary tract infection mediated by uropathogenic Escherichia coli.

doi: 10.1038/s41598-019-38691-8

Figure Lengend Snippet: Figure 6. Neutrophil migration mediated by renal fibroblasts and renal epithelial cells. Primary human renal fibroblasts (HRF) and human renal epithelial cells (A498) were stimulated with CFT073 at MOI10 for 3 hours followed by neutrophil migration over a transwell membrane. Data are presented as mean ± SEM (n = 3 independent experiments). Asterisks denote statistical significance compared to respective unstimulated control cells (***p < 0.001).

Article Snippet: Primary human renal fibroblasts (Pelobiotech GmbH, Planegg, Germany) were derived from a healthy woman and the human renal epithelial cell line A498 (HTB-44, ATCC) was derived from a kidney carcinoma.

Techniques: Migration, Membrane, Control

Journal: Scientific reports

Article Title: Human renal fibroblasts are strong immunomobilizers during a urinary tract infection mediated by uropathogenic Escherichia coli.

doi: 10.1038/s41598-019-38691-8

Figure Lengend Snippet: Figure 5. The release of inflammatory proteins from renal fibroblasts and renal epithelial cells upon a UPEC infection. Primary human renal fibroblasts (HRF) and human renal epithelial cells (A498) were stimulated with CFT073 at MOI 10 for 6 hours followed by the analysis of 92 inflammatory proteins simultaneously using the proximity extension assay on the Proseek Multiplex inflammation panel. The protein data is presented as normalized protein expression units (NPX) where a high value corresponds to high protein concentration. Data are presented as mean ± SEM (n = 3 independent experiments). Asterisks denote statistical significance compared to respective unstimulated control cells (*p < 0.05, *p < 0.01, ***p < 0.001).

Article Snippet: Primary human renal fibroblasts (Pelobiotech GmbH, Planegg, Germany) were derived from a healthy woman and the human renal epithelial cell line A498 (HTB-44, ATCC) was derived from a kidney carcinoma.

Techniques: Infection, Multiplex Assay, Expressing, Protein Concentration, Control

Journal: PLoS ONE

Article Title: Global gene expression profiling and antibiotic susceptibility after repeated exposure to the carbon monoxide-releasing molecule-2 (CORM-2) in multidrug-resistant ESBL-producing uropathogenic Escherichia coli

doi: 10.1371/journal.pone.0178541

Figure Lengend Snippet: A) IL-6 and IL-8 production from A498 renal epithelial cells after stimulation for 6 h with the original ESBL7 isolate or with ESBL7 that have been pre-exposed 20 times to CORM-2 (250 μM) or vehicle (2.5% DMSO). B) Host renal cell cytotoxicity measured as LDH-release during the same conditions as in panel A and normalized to unstimulated and lysed control cells. Data are presented as mean ± SEM from three independent experiments. Asterisk denotes statistical significance (*p<0.05).

Article Snippet: The human renal epithelial cell line A498 (HTB-44) was obtained from American Type Culture Collection (Manassas, USA) and cultured in Dulbecco's modified eagle medium (DMEM, Sigma-Aldrich) containing 10% fetal bovine serum (FBS), 2 mM L-glutamine, 1 mM non-essential amino acids (all from Invitrogen Ltd, Paisley, UK) at 37°C in a 5% CO 2 atmosphere.

Techniques: Control